Proteomic profiling of camel and cow milk proteins under heat treatment.
Identifieur interne : 000347 ( Main/Exploration ); précédent : 000346; suivant : 000348Proteomic profiling of camel and cow milk proteins under heat treatment.
Auteurs : Imène Felfoul [Tunisie] ; Julien Jardin [France] ; Frédéric Gaucheron [France] ; Hamadi Attia [Tunisie] ; M A Ayadi [Tunisie]Source :
- Food chemistry [ 1873-7072 ] ; 2017.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Bovins (MeSH), Caséines (analyse), Chameaux (MeSH), Chromatographie en phase liquide (MeSH), Lactoglobulines (analyse), Protéines de lactosérum (analyse), Protéines de lactosérum (composition chimique), Protéines de lait (analyse), Protéomique (MeSH), Spectrométrie de masse en tandem (MeSH), Température élevée (MeSH), Électrophorèse sur gel de polyacrylamide (MeSH).
- MESH :
English descriptors
- KwdEn :
- Animals (MeSH), Camelus (MeSH), Caseins (analysis), Cattle (MeSH), Chromatography, Liquid (MeSH), Electrophoresis, Polyacrylamide Gel (MeSH), Hot Temperature (MeSH), Lactoglobulins (analysis), Milk Proteins (analysis), Proteomics (MeSH), Tandem Mass Spectrometry (MeSH), Whey Proteins (analysis), Whey Proteins (chemistry).
- MESH :
- chemical , analysis : Caseins, Lactoglobulins, Milk Proteins, Whey Proteins.
- chemical , chemistry : Whey Proteins.
- Animals, Camelus, Cattle, Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Hot Temperature, Proteomics, Tandem Mass Spectrometry.
Abstract
Cow and camel milk proteins before and after heat treatment at 80°C for 60min were identified using LC/MS and LC-MS/MS following monodimensional electrophoresis. The database used for the identification of camel and cow proteins was set from http://www.uniprot.org/. The obtained results showed that, after heating, camel milk at 80°C for 60min, camel α-lactalbumin (α-la) and peptidoglycan recognition protein (PGRP) were not detected while camel serum albumin (CSA) was significantly diminished. When heating cow milk at 80°C for 60min, α-lactalbumin (α-la) and β-lactoglobulin (β-lg) were not significantly detected. Moreover, 19 protein bands from SDS-PAGE were analyzed and a total of 45 different proteins were identified by LC-MS/MS. Casein fractions were kept intact under a heat treatment of 80°C during 60min of both camel and cow milks. Camel and bovine whey proteins were affected by a heat treatment of 80°C for 60min.
DOI: 10.1016/j.foodchem.2016.08.007
PubMed: 27596405
Affiliations:
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Le document en format XML
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The database used for the identification of camel and cow proteins was set from http://www.uniprot.org/. The obtained results showed that, after heating, camel milk at 80°C for 60min, camel α-lactalbumin (α-la) and peptidoglycan recognition protein (PGRP) were not detected while camel serum albumin (CSA) was significantly diminished. When heating cow milk at 80°C for 60min, α-lactalbumin (α-la) and β-lactoglobulin (β-lg) were not significantly detected. Moreover, 19 protein bands from SDS-PAGE were analyzed and a total of 45 different proteins were identified by LC-MS/MS. Casein fractions were kept intact under a heat treatment of 80°C during 60min of both camel and cow milks. Camel and bovine whey proteins were affected by a heat treatment of 80°C for 60min. </div></front></TEI><affiliations><list><country><li>France</li><li>Tunisie</li></country><region><li>Région Bretagne</li></region></list><tree><country name="Tunisie"><noRegion><name sortKey="Felfoul, Imene" sort="Felfoul, Imene" uniqKey="Felfoul I" first="Imène" last="Felfoul">Imène Felfoul</name></noRegion><name sortKey="Attia, Hamadi" sort="Attia, Hamadi" uniqKey="Attia H" first="Hamadi" last="Attia">Hamadi Attia</name><name sortKey="Ayadi, M A" sort="Ayadi, M A" uniqKey="Ayadi M" first="M A" last="Ayadi">M A Ayadi</name></country><country name="France"><region name="Région Bretagne"><name sortKey="Jardin, Julien" sort="Jardin, Julien" uniqKey="Jardin J" first="Julien" last="Jardin">Julien Jardin</name></region><name sortKey="Gaucheron, Frederic" sort="Gaucheron, Frederic" uniqKey="Gaucheron F" first="Frédéric" last="Gaucheron">Frédéric Gaucheron</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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